Nest-o-patch

This program was designed mainly for “preconditioning” and Analysis of single channel properties in patch-clamp recordings. Single channel conductance, kinetics, NPo and open probability may be estimated. The program focused mainly on the analysis of level dwellings distribution in a recording with several channels and estimate of the number of channels (N) and open probability (Po). Analysis of hidden Markov models is planned in the future versions.

However, the program may be useful in various applications, for example, analysis of whole-cell mode recordings, or of any other data, when limited editing and measurements in a 2-dimentional (for example time-current) wave are required. The program itself does not make any assumptions about units of the recordings, it deals only with X-values and Y-values.

Directly supports files, created by PULSE software (HEKA Elektronik Dr. Schulze GmbH) and opens ASCII files.

Features

- Opens data exported into ascii format and directly reads Pulse (HEKA) files
- Loads several segments of Pulse recording at once
- Easy correction of drifting baseline
- Efficient data filtering
- Advanced analysis of channel kinetics
- Estimate of Number of channels (N) and open probability (Po) based on the analysis of level dwellings distribution
- Saves modified traces, binned current histograms and fitting results as .csv files.
- ...and much more